Translational control of recombinant human acetylcholinesterase accumulation in plants

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Translational control of recombinant human acetylcholinesterase accumulation in plants

BACKGROUND Codon usage differences are known to regulate the levels of gene expression in a species-specific manner, with the primary factors often cited to be mRNA processing and accumulation. We have challenged this conclusion by expressing the human acetylcholinesterase coding sequence in transgenic plants in its native GC-rich sequence and compared to a matched sequence with (dicotyledonous...

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Expression of recombinant human acetylcholinesterase in transgenic tomato plants.

Enzyme therapy for the prevention and treatment of organophosphate poisoning depends on the availability of large amounts of cholinesterases. Transgenic plants are being evaluated for their efficiency and cost-effectiveness as a system for the bioproduction of therapeutically valuable proteins. Here we report production of a recombinant isoform of human acetylcholinesterase in transgenic tomato...

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In many parts of the world, groundwater contains so much arsenic that it builds up in irrigated crops. Linked to cancer and heart disease, this toxic element is particularly worrisome in rice, which absorbs arsenic more readily than other grains and is a staple for billions of people. Countries with the double whammy of arsenic-laced groundwater and heavy rice consumption include Bangladesh, In...

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Acetylcholinesterase inhibitors from plants.

Inhibition of acetylcholinesterase (AChE), the key enzyme in the breakdown of acetylcholine, is considered as a promising strategy for the treatment of neurological disorders such as Alzheimer's disease, senile dementia, ataxia and myasthenia gravis. A potential source of AChE inhibitors is certainly provided by the abundance of plants in nature. This article aims to provide a comprehensive lit...

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ژورنال

عنوان ژورنال: BMC Biotechnology

سال: 2007

ISSN: 1472-6750

DOI: 10.1186/1472-6750-7-27